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Elisa has been used as a diagnostic tool in medicine, plant pathology, and biotechnology, as well as a quality control check in various industries It is a method of quantifying an antigen immobilized on a solid surface. In the most simple form of an elisa, antigens from the sample to be tested are attached to a surface.

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Learn about the different methods for performing an elisa assay for protein quantitation, including assay design strategies and reagents. Learn about elisas (enzyme linked immunosorbent assays) and the various types of elisa including direct, indirect, sandwich and competitive elisas. Elisa is a laboratory technique that detects certain antibodies, antigens and other substances in your blood, pee or other bodily fluid

Laboratory scientists use this technique for several medical tests — from diagnosing infections to confirming pregnancy.

This guide explains what an elisa (short for enzyme linked immunosorbent assay) is, the procedures involved, types of elisa, detection options and results